Department of Analytical Chemistry and Food Chemistry University of Vienna
The project aims to develop new techniques and materials to be used for studying protein modifications. These modifications (for example, the attachment of phosphate groups or sugar chains) play an important role in a multitude of biological processes like signaling in a cell or disease development.

While a number of strategies for studying post-transpational modifications have been reported in recent years, many of them are not suitable for application on highly complex samples of biological origin, such as cell lysates or body fluids. Moreover, there is strong demand for methods that not only determine the presence of these modifications (i.e. qualitative analysis) but also the degree / extent of the modifications (i.e. quantitative analysis).

The project focuses on two major fields of current interest:

• Materials and methods for the analysis of protein / peptide phosphorylation (phospho-proteomics, phospho-peptidomics)
• Immobilization chemistry for drug compounds to be used in pull-down assays (drug proteomics; protein-protein interactions)

Innovative chemical tools will be developed to improve currently available methodology in terms of specificity, sensitivity and use for quantitative assays.
Mass spectromtry in combination with liquid chromatography is the central analyitical technique that is used for development and evaluation of such new materials. These include, for example, metal oxide sorbents for selective enrichment of phospho-peptides and improved performance over existing technology (such as immobilized metal affinity chromatography).

The Department of Analytical Chemistry and Food Chemistry, where the research group is located, is part of the Faculty of Chemistry at the University of Vienna. Main research areas of the group of Univ.-Prof. Dr. Wolfgang Lindner includes:

1.) the study of molecular recognition concepts on a broad basis

2.) the development of highly selective sorption materials and their applications to the fields of separation
science and

3.) mass spectrometry

Numerous cooperations exist on the acedemic leval as well as with industry. The Christian Doppler Laboratory for Molecular Recognition Materials, an R&D collaboration between academia and industry partners, is also part of the research group.

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